Quickstart#

from pykingenie import *

bli = OctetExperiment('test')
# Replace with your own folder containing .frd files !!! The .frd files can be exported from the Octet software
folder = '/home/os/spc_shiny_servers/kineticsApp/appFiles/KinGenie/www/test_bli_folder/'
files = os.listdir(folder)
files = [folder + file for file in files]

files.sort()

bli.read_sensor_data(files)

bli.align_association(bli.sensor_names)
bli.align_dissociation(bli.sensor_names)

# We use the sensor #8 as the reference sensor for baseline correction. Remember than python indexing starts at 0.
bli.subtraction(bli.sensor_names[:7], bli.sensor_names[7])

pyKinetics = KineticsAnalyzer()
pyKinetics.add_experiment(bli, 'test')

labels = pyKinetics.get_experiment_properties('sensor_names')
ids    = pyKinetics.get_experiment_properties('sensor_names_unique')

# Flatten the lists
labels = [item for sublist in labels for item in sublist]
ids    = [item for sublist in ids for item in sublist]

df = get_plotting_df(ids, labels)

figure = plot_traces_all(pyKinetics,df)
figure.write_image('test_bli_traces.png')

pyKinetics.merge_ligand_conc_df()
pyKinetics.init_fittings()

pyKinetics.generate_fittings(pyKinetics.combined_ligand_conc_df)

fig = plot_association_dissociation(pyKinetics,split_by_smax_id=True)
fig.write_image('test_bli.png')

pyKinetics.submit_steady_state_fitting()
pyKinetics.submit_kinetics_fitting()

fig = plot_association_dissociation(pyKinetics)
fig.write_image('test_bli_fitted.png')